|
KMID : 1149220130210010071
|
|
Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 1 p.71 ~ p.79
|
|
|
Simultaneous Analysis of Six Compounds in Yijin-tang by HPLC-PDA
|
|
Kim Seong-Sil
Kim Jung-Hoon
Shin Hyeun-Kyoo
Seo Chang-Seob
|
|
|
Abstract
|
|
|
Objectives : Yijin-tang has been used in the treatment of gastrointestinal diseases such as irritable bowel syndrome, gastritis, and gastric ulcer. In this study, a high-performance liquid chromatography (HPLC) method was established for simultaneous analysis of six compounds, liquiritin, glycyrrhizin, hesperidin, 6-gingerol, homogentisic acid, and 3,4-dihydroxybenzaldehyde in Yijin-tang, a traditional Korean herbal medicinal preparation.
Methods : A Gemini C18 column was used for the separation of six constituents at 40℃. The mobile phase using gradient elution consist of two solvent systems, 1.0% acetic acid in water (A) and 1.0% acetic acid in acetonitrile (B). The flow-rate was 1.0 mL/min and injection volume was 10 μg. The detector was a photodiode array (PDA) detector set at 254 nm and 280 nm.
Results : The calibration curves of six compounds showed good linearity in various concentration ranges (R² ≥0.9997). The limits of detection (LOD) and limits of quantification (LOQ) were 0.028?0.192 μg/mL and 0.093 ?0.540μg/mL, respectively. The RSD (%) of the intra and inter day validations were 0.03?0.84% and 0.05 ?1.00%, respectively. Recovery was 96.14?01.90% and RSD (%) was less than 1.5%.
Conclusions : The established simultaneous analysis methods will help management to improve the quality of Yijin-tang.
|
|
|
KEYWORD
|
|
|
Yijin-tang, HPLC-PDA, Validation, Simultaneous determination
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|